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Addgene

pKAd5f11pES-PmeI
(Plasmid #122698)

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 122698 Standard format: Plasmid sent in bacteria as agar stab 1 $85

Backbone

  • Vector backbone
    the genome of E1/E3 deleted human adenovirus 5
  • Backbone manufacturer
    Stratagene
  • Vector type
    Adenoviral

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    30°C
  • Growth Strain(s)
    NEB Stable
  • Growth instructions
    Other growth strain: TOP10
  • Copy number
    High Copy

Cloning Information

  • Cloning method Gibson Cloning

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

This plasmid contains an in frame deletion of R346 and R347 from the L2 pV open reading frame. This mutation is not expected to affect its function.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pKAd5f11pES-PmeI was a gift from Zhuo-Zhuang Lu (Addgene plasmid # 122698 ; http://n2t.net/addgene:122698 ; RRID:Addgene_122698)
  • For your References section:

    Single plasmid-based, upgradable and backward compatible adenoviral vector systems. Liu H, Lu Z, Zhang X, Guo X, Mei L, Zou X, Zhong Y, Wang M, Hung T. Hum Gene Ther. 2019 Feb 22. doi: 10.1089/hum.2018.258. 10.1089/hum.2018.258 PubMed 30793964