pKAd5f11p-EPG
(Plasmid
#122697)
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PurposeTo generate E1/E3-deleted human adenovirus 5 (HAdV-5) bearing a chimeric fiber and an EF1a promoter-controlled transgene.
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 122697 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonethe genome of E1/E3 deleted human adenovirus 5
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Backbone manufacturerStratagene
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Vector typeAdenoviral
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature30°C
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Growth Strain(s)NEB Stable
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Growth instructionsOther growth strain: TOP10
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameGFP
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SpeciesSynthetic
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Insert Size (bp)743
- Promoter EF1a
Cloning Information
- Cloning method Gibson Cloning
- 5′ sequencing primer Unknown (Common Sequencing Primers)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
This plasmid contains an in frame deletion of R346 and R347 from the L2 pV open reading frame. This mutation is not expected to affect its function.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pKAd5f11p-EPG was a gift from Zhuo-Zhuang Lu (Addgene plasmid # 122697 ; http://n2t.net/addgene:122697 ; RRID:Addgene_122697) -
For your References section:
Single plasmid-based, upgradable and backward compatible adenoviral vector systems. Liu H, Lu Z, Zhang X, Guo X, Mei L, Zou X, Zhong Y, Wang M, Hung T. Hum Gene Ther. 2019 Feb 22. doi: 10.1089/hum.2018.258. 10.1089/hum.2018.258 PubMed 30793964