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pcDNA3.1-V5H6-mPCSK3 D153A
(Plasmid #122681)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 122681 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pcDNA3.1/V5-His
  • Backbone manufacturer
    Invitrogen
  • Backbone size w/o insert (bp) 5428
  • Total vector size (bp) 7809
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Mus musculus furin (paired basic amino acid cleaving enzyme) (Furin), transcript variant 1
  • Species
    M. musculus (mouse)
  • Insert Size (bp)
    2381
  • Mutation
    changed Aspartic Acid 153 to Alanine
  • GenBank ID
    NM_011046.3
  • Entrez Gene
    Furin (a.k.a. 9130404I01Rik, Fur, PACE, Pcsk3, SPC1)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pcDNA3.1-V5H6-mPCSK3 D153A was a gift from Liming Pei (Addgene plasmid # 122681 ; http://n2t.net/addgene:122681 ; RRID:Addgene_122681)

  • For your References section:

    Growth Differentiation Factor 15 Maturation Requires Proteolytic Cleavage by PCSK3, -5, and -6. Li JJ, Liu J, Lupino K, Liu X, Zhang L, Pei L. Mol Cell Biol. 2018 Oct 15;38(21). pii: MCB.00249-18. doi: 10.1128/MCB.00249-18. Print 2018 Nov 1. 10.1128/MCB.00249-18 PubMed 30104250
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