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Addgene

pcDNA3.1-mPCSK8
(Plasmid #122677)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 122677 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pcDNA3.1
  • Backbone size w/o insert (bp) 5428
  • Total vector size (bp) 8586
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Mus musculus membrane-bound transcription factor peptidase, site 1 (Mbtps1), transcript variant 2
  • Insert Size (bp)
    3158
  • GenBank ID
    NM_001167910.1
  • Entrez Gene
    Mbtps1 (a.k.a. 0610038M03Rik, AV003995, S1P, SKI-1, Ski1, mKIAA0091)
  • Promoter T7

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRV (not destroyed)
  • 3′ cloning site XhoI (not destroyed)
  • 5′ sequencing primer GTA ATA CGA CTC ACT ATA GGG
  • 3′ sequencing primer TAG AAG GCA CAG TCG AGG
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pcDNA3.1-mPCSK8 was a gift from Liming Pei (Addgene plasmid # 122677 ; http://n2t.net/addgene:122677 ; RRID:Addgene_122677)

  • For your References section:

    Growth Differentiation Factor 15 Maturation Requires Proteolytic Cleavage by PCSK3, -5, and -6. Li JJ, Liu J, Lupino K, Liu X, Zhang L, Pei L. Mol Cell Biol. 2018 Oct 15;38(21). pii: MCB.00249-18. doi: 10.1128/MCB.00249-18. Print 2018 Nov 1. 10.1128/MCB.00249-18 PubMed 30104250
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