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Addgene

LisDPS
(Plasmid #122654)

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 122654 Standard format: Plasmid sent in bacteria as agar stab 1 $85

Backbone

  • Vector backbone
    pET30a
  • Backbone manufacturer
    Novagen
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Growth instructions
    Use bacterial strain BL21(DE3) for expression.
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    DPS, listeria
  • Species
    Listeria innocua

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site unknown (unknown if destroyed)
  • 3′ cloning site unknown (unknown if destroyed)
  • 5′ sequencing primer n/a
  • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

This plasmid is also used in the following publications:
Resnick, D. A., Gilmore, K., Idzerda, Y. U., Klem, M. T., Allen, M., Douglas, T., … Young, M. (2006). Magnetic properties of Co 3O 4 nanoparticles mineralized in Listeria innocua Dps. Journal of Applied Physics, 99(8), 1–4. https://doi.org/10.1063/1.2163839

Okuda, M., Suzumoto, Y., Iwahori, K., Kang, S., Uchida, M., Douglas, T., & Yamashita, I. (2010). Bio-templated CdSe nanoparticle synthesis in a cage shaped protein, Listeria-Dps, and their two dimensional ordered array self-assembly. Chemical Communications, 46(46), 8797–8799. https://doi.org/10.1039/c0cc03298k

Usselman, R. J., Russek, S. E., Klem, M. T., Allen, M. A., Douglas, T., Young, M., … Singel, D. J. (2012). Temperature dependence of electron magnetic resonance spectra of iron oxide nanoparticles mineralized in Listeria innocua protein cages. Journal of Applied Physics, 112(8), 1–9. https://doi.org/10.1063/1.4757964

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    LisDPS was a gift from Trevor Douglas (Addgene plasmid # 122654 ; http://n2t.net/addgene:122654 ; RRID:Addgene_122654)
  • For your References section:

    Constrained synthesis of cobalt oxide nanomaterials in the 12-subunit protein cage from Listeria innocua. Allen M, Willits D, Young M, Douglas T. Inorg Chem. 2003 Oct 6;42(20):6300-5. doi: 10.1021/ic0343657. 10.1021/ic0343657 PubMed 14514305