LisDPS
(Plasmid
#122654)
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PurposeExpression of LisDPS in bacterial cells
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 122654 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepET30a
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Backbone manufacturerNovagen
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Growth instructionsUse bacterial strain BL21(DE3) for expression.
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameDPS, listeria
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SpeciesListeria innocua
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site unknown (unknown if destroyed)
- 3′ cloning site unknown (unknown if destroyed)
- 5′ sequencing primer n/a (Common Sequencing Primers)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
This plasmid is also used in the following publications:
Resnick, D. A., Gilmore, K., Idzerda, Y. U., Klem, M. T., Allen, M., Douglas, T., … Young, M. (2006). Magnetic properties of Co 3O 4 nanoparticles mineralized in Listeria innocua Dps. Journal of Applied Physics, 99(8), 1–4. https://doi.org/10.1063/1.2163839
Okuda, M., Suzumoto, Y., Iwahori, K., Kang, S., Uchida, M., Douglas, T., & Yamashita, I. (2010). Bio-templated CdSe nanoparticle synthesis in a cage shaped protein, Listeria-Dps, and their two dimensional ordered array self-assembly. Chemical Communications, 46(46), 8797–8799. https://doi.org/10.1039/c0cc03298k
Usselman, R. J., Russek, S. E., Klem, M. T., Allen, M. A., Douglas, T., Young, M., … Singel, D. J. (2012). Temperature dependence of electron magnetic resonance spectra of iron oxide nanoparticles mineralized in Listeria innocua protein cages. Journal of Applied Physics, 112(8), 1–9. https://doi.org/10.1063/1.4757964
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
LisDPS was a gift from Trevor Douglas (Addgene plasmid # 122654 ; http://n2t.net/addgene:122654 ; RRID:Addgene_122654) -
For your References section:
Constrained synthesis of cobalt oxide nanomaterials in the 12-subunit protein cage from Listeria innocua. Allen M, Willits D, Young M, Douglas T. Inorg Chem. 2003 Oct 6;42(20):6300-5. doi: 10.1021/ic0343657. 10.1021/ic0343657 PubMed 14514305