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Mm-PylRS-AF/Pyl-tRNACUA
(Plasmid #122650)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 122650 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pCMV
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    PylRS-AF, Pyl-tRNACUA
  • Species
    Methanosarcina mazei
  • Mutation
    Tyrosine 306 changed to Alanine, Tyrosine 384 changed to Phenylalanine
  • Tag / Fusion Protein
    • FLAG

Cloning Information

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    Prof. Peng R. Chen, Peking University, China
  • Articles Citing this Plasmid

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    Mm-PylRS-AF/Pyl-tRNACUA was a gift from Howard Hang (Addgene plasmid # 122650 ; http://n2t.net/addgene:122650 ; RRID:Addgene_122650)

  • For your References section:

    IFITM3 directly engages and shuttles incoming virus particles to lysosomes. Spence JS, He R, Hoffmann HH, Das T, Thinon E, Rice CM, Peng T, Chandran K, Hang HC. Nat Chem Biol. 2019 Jan 14. pii: 10.1038/s41589-018-0213-2. doi: 10.1038/s41589-018-0213-2. 10.1038/s41589-018-0213-2 [pii] PubMed 30643282
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