pET28b-FOXP2_P3
(Plasmid
#122647)
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PurposeExpresses truncated variant (aa367-aa714) of human FOXP2 in bacterial cells
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 122647 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepET28b
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Backbone manufacturerNovagen
- Backbone size w/o insert (bp) 5269
- Total vector size (bp) 6352
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameHomo sapiens forkhead box P2
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Alt nameFOXP2
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SpeciesH. sapiens (human)
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Insert Size (bp)1083
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GenBank ID93986
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Entrez GeneFOXP2 (a.k.a. CAGH44, SPCH1, TNRC10)
- Promoter T7
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Tags
/ Fusion Proteins
- His-tag (N terminal on backbone)
- Strep-tag II (C terminal on insert)
Cloning Information
- Cloning method Unknown
- 5′ sequencing primer T7 promoter
- 3′ sequencing primer T7 terminator (Common Sequencing Primers)
Resource Information
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A portion of this plasmid was derived from a plasmid made byEurofins Genomics GmbH - Gene synthesis of full-lengths human FOXP2 gene
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pET28b-FOXP2_P3 was a gift from Hendrik Dietz (Addgene plasmid # 122647 ; http://n2t.net/addgene:122647 ; RRID:Addgene_122647) -
For your References section:
Dissecting FOXP2 Oligomerization and DNA Binding. Haussermann K, Young G, Kukura P, Dietz H. Angew Chem Int Ed Engl. 2019 Jun 3;58(23):7662-7667. doi: 10.1002/anie.201901734. Epub 2019 Apr 29. 10.1002/anie.201901734 PubMed 30887622