pASR103
(Plasmid
#122637)
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Purpose(Empty Backbone) BglFusion compatible IPTG inducible integration vector for Streptococcus pneumoniae (pPEPZ Backbone)
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 122637 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepPEPZ-Plac
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Backbone manufacturerJan-Willem Veening
- Backbone size (bp) 4314
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Modifications to backboneReplacement of spectinomycin marker with erythromycin marker
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Vector typeBacterial Expression, Synthetic Biology
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Growth instructionsAmpicillin marker for selection in E. coli (100 ug/ml) and erythromycin marker for selection in S. pneumoniae (0.5 ug/ml)
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Copy numberHigh Copy
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pASR103 was a gift from Jan-Willem Veening (Addgene plasmid # 122637 ; http://n2t.net/addgene:122637 ; RRID:Addgene_122637) -
For your References section:
Three New Integration Vectors and Fluorescent Proteins for Use in the Opportunistic Human Pathogen Streptococcus pneumoniae. Keller LE, Rueff AS, Kurushima J, Veening JW. Genes (Basel). 2019 May 22;10(5). pii: genes10050394. doi: 10.3390/genes10050394. 10.3390/genes10050394 PubMed 31121970
Map uploaded by the depositor.
