pPEPY-Plac
(Plasmid
#122634)
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Purpose(Empty Backbone) BglFusion compatible IPTG inducible integration vector for Streptococcus pneumoniae
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 122634 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepPEPY
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Backbone manufacturerJan-Willem Veening
- Backbone size (bp) 3329
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Modifications to backboneAddition of IPTG inducible promoter to backbone
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Vector typeBacterial Expression, Synthetic Biology
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Growth instructionsKanamycin selection for E. coli (50 ug/ml) and S. pneumoniae (250 ug/ml)
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Copy numberHigh Copy
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pPEPY-Plac was a gift from Jan-Willem Veening (Addgene plasmid # 122634 ; http://n2t.net/addgene:122634 ; RRID:Addgene_122634) -
For your References section:
Three New Integration Vectors and Fluorescent Proteins for Use in the Opportunistic Human Pathogen Streptococcus pneumoniae. Keller LE, Rueff AS, Kurushima J, Veening JW. Genes (Basel). 2019 May 22;10(5). pii: genes10050394. doi: 10.3390/genes10050394. 10.3390/genes10050394 PubMed 31121970
Map uploaded by the depositor.
