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Depositing Lab
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Publication
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 12263 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepCMVR8.2
- Backbone size w/o insert (bp) 8128
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Vector typeMammalian Expression, Lentiviral ; Packaging
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)NEB Stable
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameHIV-1 GAG/POL, Tat and Rev
Cloning Information
- Cloning method Restriction Enzyme
- 5′ sequencing primer CMV forward (Common Sequencing Primers)
Resource Information
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Supplemental Documents
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Articles Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Packaging plasmid.
Please note that the full sequence for this plasmid is approximated and not fully verified. Please visit the Trono lab http://tronolab.epfl.ch for cloning strategies, protocols, publications, and more.
Addgene Note: This is a lentiviral packaging plasmid that may contain regulatory and accessory HIV genes. Please see Naldini, et al., PNAS 1996 for more details on this construct.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pCMV delta R8.2 was a gift from Didier Trono (Addgene plasmid # 12263 ; http://n2t.net/addgene:12263 ; RRID:Addgene_12263)