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Addgene

TC476
(Plasmid #122629)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 122629 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pPD95.77
  • Vector type
    Worm Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    rtetR-QFAD::P2A::mKate::T2A::tetR-pie1
  • Alt name
    Punc-54::rtetR-QFAD::P2A::mKate::T2A::tetR-pie1
  • Species
    C. elegans (nematode)

Cloning Information

  • Cloning method Unknown

Resource Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Several differences are present in the Addgene NGS sequence compared to the theoretical sequence present in the attached Genbank file that do not impact the function of the plasmid.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    TC476 was a gift from Tian Chi (Addgene plasmid # 122629 ; http://n2t.net/addgene:122629 ; RRID:Addgene_122629)

  • For your References section:

    A Tet/Q Hybrid System for Robust and Versatile Control of Transgene Expression in C. elegans. Mao S, Qi Y, Zhu H, Huang X, Zou Y, Chi T. iScience. 2019 Jan 25;11:224-237. doi: 10.1016/j.isci.2018.12.023. Epub 2018 Dec 27. 10.1016/j.isci.2018.12.023 PubMed 30634168
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