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Addgene

pBT137-splitD
(Plasmid #122606)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 122606 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pBT137
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    M13 bacteriophage genes

Cloning Information

  • Cloning method Gibson Cloning

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

low copy vector, confers a growth defect

Please see https://benchling.com/s/seq-xGxHsGdVAtUUgfQsV2La for additional information.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pBT137-splitD was a gift from David Liu (Addgene plasmid # 122606 ; http://n2t.net/addgene:122606 ; RRID:Addgene_122606)

  • For your References section:

    Continuous evolution of base editors with expanded target compatibility and improved activity. Thuronyi BW, Koblan LW, Levy JM, Yeh WH, Zheng C, Newby GA, Wilson C, Bhaumik M, Shubina-Oleinik O, Holt JR, Liu DR. Nat Biotechnol. 2019 Jul 22. pii: 10.1038/s41587-019-0193-0. doi: 10.1038/s41587-019-0193-0. 10.1038/s41587-019-0193-0 PubMed 31332326
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