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Addgene

pBT120b
(Plasmid #122604)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 122604 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pBT120
  • Vector type
    Synthetic Biology

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    gIII, luxAB, guide RNA

Cloning Information

  • Cloning method Gibson Cloning

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Please see https://benchling.com/s/seq-I9q2W3bzpjsuOoxQPyCj for additional information.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pBT120b was a gift from David Liu (Addgene plasmid # 122604 ; http://n2t.net/addgene:122604 ; RRID:Addgene_122604)

  • For your References section:

    Continuous evolution of base editors with expanded target compatibility and improved activity. Thuronyi BW, Koblan LW, Levy JM, Yeh WH, Zheng C, Newby GA, Wilson C, Bhaumik M, Shubina-Oleinik O, Holt JR, Liu DR. Nat Biotechnol. 2019 Jul 22. pii: 10.1038/s41587-019-0193-0. doi: 10.1038/s41587-019-0193-0. 10.1038/s41587-019-0193-0 PubMed 31332326
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