psPAX2
(Plasmid #12260)

• Purpose: (Empty Backbone) 2nd generation lentiviral packaging plasmid. Can be used with 2nd or 3rd generation lentiviral vectors and envelope expressing plasmid (Addgene#12259)
• Depositing Lab: Didier Trono
• Publication: Trono Lab Packaging and Envelope Plasmids (unpublished)

Backbone

• Vector backbone: psPAX2
• Backbone size (bp): 10703
• Vector type: Mammalian Expression, Lentiviral ; Packaging

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: None

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ sequencing primer: See map

Resource Information

• Supplemental Documents:
  • BamHI and EcoRI digests
• Articles Citing this Plasmid:
  • 5151 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

2nd generation packaging vector. Known to work with many of the Aebischer and Trono lab lentiviral vectors, as well as the pLKO system. Produces higher titer than pCMV-dR8.2 dvpr.

Note: This plasmid may run as a dimer. Try testing multiple colonies to select the monomer. It may be helpful to follow protocols for low copy plasmids.

Please visit the Trono lab http://tronolab.epfl.ch for cloning strategies, protocols, publications, and more. See LentiWeb http://www.lentiweb.com for discussion on cloning strategies and protocols.