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pTn7·X-nia
(Plasmid #122592)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 122592 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pTn7-M
  • Backbone size w/o insert (bp) 3147
  • Total vector size (bp) 5856
  • Vector type
    Bacterial Expression, Synthetic Biology ; Tn7 genomic integration
  • Selectable markers
    Gentamicin

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    Pir1
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    xylS/Pm->Nia
  • Alt name
    XylS regulator
  • Alt name
    nuclear inclusion proteins a
  • Species
    Pseudomonas putida, peanut stripe potyvirus (PStV)
  • Insert Size (bp)
    2775
  • Promoter Pm

Cloning Information

  • Cloning method Ligation Independent Cloning
  • 5′ sequencing primer AGGGCGGCGGATTTGTCC
  • 3′ sequencing primer GCGGCAACCGAGCGTTC
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pTn7·X-nia was a gift from Pablo Ivan Nikel (Addgene plasmid # 122592 ; http://n2t.net/addgene:122592 ; RRID:Addgene_122592)

  • For your References section:

    Physical decoupling of XylS/Pm regulatory elements and conditional proteolysis enable precise control of gene expression in Pseudomonas putida. Volke DC, Turlin J, Mol V, Nikel PI. Microb Biotechnol. 2019 Mar 12. doi: 10.1111/1751-7915.13383. 10.1111/1751-7915.13383 PubMed 30864281
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