pS2513·PHP
(Plasmid #122590)

• Purpose: Constitutive expressed pHiorine2 for pH quan. in gram-negative cells
• Depositing Lab: Pablo Ivan Nikel
• Publication: Arce-Rodriguez et al Microb Biotechnol. 2019 Jun 4. doi: 10.1111/1751-7915.13439.

Backbone

• Vector backbone: pSEVA2513
• Backbone manufacturer: SEVA
• Backbone size w/o insert (bp): 5300
• Total vector size (bp): 6013
• Vector type: Bacterial Expression, Synthetic Biology

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Low Copy

Gene/Insert

• Gene/Insert name: phIorine2
• Species: synthetic, based on superfolder GFP
• Insert Size (bp): 717
• Promoter: Pem7

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: HindIII (not destroyed)
• 3′ cloning site: SpeI (not destroyed)
• 5′ sequencing primer: AGGGCGGCGGATTTGTCC
• 3′ sequencing primer: GCGGCAACCGAGCGTTC

Resource Information

• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry