pMD2.G Notes
Addgene diagnostic digest- NotI, HindIII
This gel depicts the NEB 1kb ladder and a digestion of the plasmid with the following enzymes: NotI, HindIII and NotI+HindIII double digest.
We expected the following band sizes for this digest: 5.8kb; 5.8kb; 3kb and 2.8kb. We see the expected pattern.
Addgene Diagnostic Digest- BamHI
This gel depicts the NEB 1kb ladder and a digestion of the plasmid pMD2.G with the following enzymes: BamHI-HF.
Expected Bands
BamHI-HF : 5 kb, .8 kb & .031 kb (off gel)
We observe the expected bands for this digest.
Addgene Diagnostic Digest- PvuI-HF and ApaI
This gel depicts the NEB 1kb ladder and a digestion of the plasmid pMD2.G with the following enzymes: PvuI-HF and ApaI.
Expected Bands:
PvuI-HF: 5822 bp
ApaI: 5822 bp
We observe the expected bands for this digest.
Addgene Diagnostic Digest- EcoRI
This gel depicts the NEB 1kb ladder and a digestion of the plasmid pMD2.G with the following enzymes: EcoRI-HF.
Expected bands
EcoRI-HF: 4154 bp & 1668 bp
We observe the expected bands for this digest.
Addgene Diagnostic Digest
This gel depicts the NEB 1kb Ladder and a digestion of the plasmid pMD2.G with the following enzymes: HindIII-HF, PvuI-HF
Expected bands:
HindIII-HF/PvuI-HF: Two bands: 4429 bp and 1393 bp
Results:
We observed the expected bands for this digest.