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Addgene

Human Inositol 1,4,5-Trisphosphate 3-Kinase B
(Plasmid #122589)

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 122589 Standard format: Plasmid sent in bacteria as agar stab 1 $85

Backbone

  • Vector backbone
    pcDNA3
  • Backbone manufacturer
    Invitrogen
  • Backbone size w/o insert (bp) 5428
  • Total vector size (bp) 8435
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    inositol 1,4,5-trisphosphate 3-kinase B
  • Alt name
    ITPKB
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    3007
  • GenBank ID
    CAB65055.3
  • Entrez Gene
    ITPKB (a.k.a. IP3-3KB, IP3K, IP3K-B, IP3KB, PIG37)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site KpnI (not destroyed)
  • 3′ cloning site XhoI (not destroyed)
  • 5′ sequencing primer CMV-F
  • 3′ sequencing primer BGH-rev
  • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    Human Inositol 1,4,5-Trisphosphate 3-Kinase B was a gift from Christophe Erneux (Addgene plasmid # 122589 ; http://n2t.net/addgene:122589 ; RRID:Addgene_122589)
  • For your References section:

    Cloning and expression of a full-length cDNA encoding human inositol 1,4,5-trisphosphate 3-kinase B. Dewaste V, Roymans D, Moreau C, Erneux C. Biochem Biophys Res Commun. 2002 Feb 22;291(2):400-5. doi: 10.1006/bbrc.2002.6456. 10.1006/bbrc.2002.6456 PubMed 11846419