Human Inositol 1,4,5-Trisphosphate 3-Kinase B
(Plasmid
#122589)
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PurposeExpression of human inositol 1,4,5-trisphosphate 3-kinase B
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 122589 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepcDNA3
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Backbone manufacturerInvitrogen
- Backbone size w/o insert (bp) 5428
- Total vector size (bp) 8435
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameinositol 1,4,5-trisphosphate 3-kinase B
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Alt nameITPKB
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SpeciesH. sapiens (human)
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Insert Size (bp)3007
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GenBank IDCAB65055.3
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Entrez GeneITPKB (a.k.a. IP3-3KB, IP3K, IP3K-B, IP3KB, PIG37)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site KpnI (not destroyed)
- 3′ cloning site XhoI (not destroyed)
- 5′ sequencing primer CMV-F
- 3′ sequencing primer BGH-rev (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
Human Inositol 1,4,5-Trisphosphate 3-Kinase B was a gift from Christophe Erneux (Addgene plasmid # 122589 ; http://n2t.net/addgene:122589 ; RRID:Addgene_122589) -
For your References section:
Cloning and expression of a full-length cDNA encoding human inositol 1,4,5-trisphosphate 3-kinase B. Dewaste V, Roymans D, Moreau C, Erneux C. Biochem Biophys Res Commun. 2002 Feb 22;291(2):400-5. doi: 10.1006/bbrc.2002.6456. 10.1006/bbrc.2002.6456 PubMed 11846419