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Addgene

pGT-B2
(Plasmid #122580)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 122580 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pJN105
  • Backbone size w/o insert (bp) 5400
  • Total vector size (bp) 6232
  • Modifications to backbone
    gent resistance replaced with beta-lactamase
  • Vector type
    Bacterial Expression, Synthetic Biology

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    sfGFP
  • Species
    Synthetic
  • Insert Size (bp)
    898

Cloning Information

  • Cloning method Gibson Cloning
  • 5′ sequencing primer CTATGCTACTCCGTCAAGCC
  • 3′ sequencing primer CGCTTACAATTTCCATTCGCCATTC
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

See supplemental tables of the referenced paper for more information on each plasmid.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pGT-B2 was a gift from Harris Wang (Addgene plasmid # 122580 ; http://n2t.net/addgene:122580 ; RRID:Addgene_122580)

  • For your References section:

    Metagenomic engineering of the mammalian gut microbiome in situ. Ronda C, Chen SP, Cabral V, Yaung SJ, Wang HH. Nat Methods. 2019 Feb;16(2):167-170. doi: 10.1038/s41592-018-0301-y. Epub 2019 Jan 14. 10.1038/s41592-018-0301-y PubMed 30643213
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