pGEM-T Easy-Gbait-hs-QF2
(Plasmid #122563)

• Purpose: Integration of QF2 sequence using the CRISPR/Cas9 system.
• Depositing Lab: Marnie Halpern
• Publication: Choi et al Elife. 2021 Dec 8;10:e72345. doi: 10.7554/eLife.72345.

Backbone

• Vector backbone: pGEM-T Easy
• Total vector size (bp): 5051
• Vector type: Cloning

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Gbait-hsp70-QF2-SV40pA
• Species: D. rerio (zebrafish)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: SacII (not destroyed)
• 3′ cloning site: SacII (not destroyed)
• 5′ sequencing primer: T7
• 3′ sequencing primer: SP6

Resource Information

• Supplemental Documents:
  • 1438. Gb-hs-QF2_pGEM T-easy.gb
• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

This plasmid was constructed by modifying the Gbait-hsp70:Gal4 donor plasmid described in Kimura et al. “Efficient generation of knock-in transgenic zebrafish carrying reporter/driver genes by CRISPR/Cas9-mediated genome engineering” Scientific reports vol. 4 6545. 8 Oct. 2014, doi:10.1038/srep06545. The QF2 sequence used was described in Riabinina et al. “Improved and expanded Q-system reagents for genetic manipulations” Nature methods vol. 12,3 (2015): 219-22, 5 p following 222.