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Addgene

pGEM-T Easy-Gbait-hs-Cre
(Plasmid #122562)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 122562 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pGEM-T Easy
  • Total vector size (bp) 4996
  • Vector type
    Cloning

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Gbait-hsp70-Cre-SV40pA
  • Species
    D. rerio (zebrafish)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site SacII (not destroyed)
  • 3′ cloning site SacII (not destroyed)
  • 5′ sequencing primer T7
  • 3′ sequencing primer SP6
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

This plasmid was constructed by modifying the Gbait-hsp70:Gal4 donor plasmid described in Kimura et al. “Efficient generation of knock-in transgenic zebrafish carrying reporter/driver genes by CRISPR/Cas9-mediated genome engineering” Scientific reports vol. 4 6545. 8 Oct. 2014, doi:10.1038/srep06545.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pGEM-T Easy-Gbait-hs-Cre was a gift from Marnie Halpern (Addgene plasmid # 122562 ; http://n2t.net/addgene:122562 ; RRID:Addgene_122562)

  • For your References section:

    Specialized neurons in the right habenula mediate response to aversive olfactory cues. Choi JH, Duboue ER, Macurak M, Chanchu JM, Halpern ME. Elife. 2021 Dec 8;10:e72345. doi: 10.7554/eLife.72345. 10.7554/eLife.72345 PubMed 34878403
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