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Addgene

pBT3035
(Plasmid #122543)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 122543 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pPD49.26
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Pnhx-2_lmn-1::CemOrange2
  • Species
    C. elegans (nematode), Synthetic
  • Entrez Gene
    lmn-1 (a.k.a. CELE_DY3.2)
  • Promoter Pnhx-2

Cloning Information

  • Cloning method Gibson Cloning

Resource Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pBT3035 was a gift from Gary Silverman (Addgene plasmid # 122543 ; http://n2t.net/addgene:122543 ; RRID:Addgene_122543)

  • For your References section:

    CemOrange2 fusions facilitate multifluorophore subcellular imaging in C. elegans. Thomas BJ, Wight IE, Chou WYY, Moreno M, Dawson Z, Homayouni A, Huang H, Kim H, Jia H, Buland JR, Wambach JA, Cole FS, Pak SC, Silverman GA, Luke CJ. PLoS One. 2019 Mar 26;14(3):e0214257. doi: 10.1371/journal.pone.0214257. eCollection 2019. 10.1371/journal.pone.0214257 PubMed 30913273
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