pRSV-Rev
(Plasmid #12253)

• Purpose: 3rd generation lentiviral packaging plasmid; Contains Rev; also requires pMDLg/pRRE (Addgene#12251) and envelope expressing plasmid (Addgene#12259)
• Depositing Lab: Didier Trono
• Publication: Dull et al J Virol. 1998 Nov . 72(11):8463-71.

Backbone

• Vector backbone: pRSV-Rev
• Backbone size w/o insert (bp): 4174
• Vector type: Mammalian Expression, Lentiviral ; Packaging

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Rev
• Entrez Gene: rev (a.k.a. HIV1gp6)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ sequencing primer: pREP fwd

Resource Information

• Addgene Notes:
  • Addgene diagnostic digest
  • Addgene Diagnostic Digest
• Articles Citing this Plasmid:
  • 771 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Rev cDNA expressing plasmid in which the joined second and third exons of HIV-1 rev are under the transcriptional control of RSV U3 promoter.

Part of the 3rd generation packaging system. Please note that most of Trono lab lentiviral vectors can only be used with the 2nd generation packaging system. See notes for pMDLg/pRRE.

Please visit the Trono lab http://tronolab.epfl.ch for cloning strategies, protocols, publications, and more. See LentiWeb http://www.lentiweb.com for discussions on cloning strategies and protocols.