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pETMCSI-T4P_Y320A
(Plasmid #122522)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 122522 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pETMCSI
  • Backbone size w/o insert (bp) 4675
  • Total vector size (bp) 7340
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    gp43 - DNA polymerase
  • Species
    Escherichia virus T4
  • Insert Size (bp)
    2679
  • Mutation
    tyrosine 320 changed to alanine
  • GenBank ID
    PubMed 12626685
  • Entrez Gene
    43 (a.k.a. T4p047)
  • Promoter T7 promoter

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NdeI (not destroyed)
  • 3′ cloning site EcoRI (not destroyed)
  • 5′ sequencing primer T7 forward
  • 3′ sequencing primer T7 reverse
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pETMCSI-T4P_Y320A was a gift from Gottfried Otting (Addgene plasmid # 122522 ; http://n2t.net/addgene:122522 ; RRID:Addgene_122522)

  • For your References section:

    Mutant T4 DNA polymerase for easy cloning and mutagenesis. Qi R, Otting G. PLoS One. 2019 Jan 23;14(1):e0211065. doi: 10.1371/journal.pone.0211065. eCollection 2019. PONE-D-18-25950 [pii] PubMed 30673756
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