pRRLSIN.cPPT.PGK-GFP.WPRE
(Plasmid #12252)

• Purpose: (Empty Backbone) 3rd generation lentiviral backbone
• Depositing Lab: Didier Trono
• Publication: Trono Lab Misc Plasmids (unpublished)

Backbone

• Vector backbone: pRRL
• Backbone manufacturer: Naldini lab
• Vector type: Mammalian Expression, Lentiviral

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): NEB Stable
• Growth instructions: Use Stbl3 or HB101 to reduce chance of recombination. Grow at 37C
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: None

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ sequencing primer: See map

Resource Information

• Addgene Notes:
  • Addgene diagnostic digest
• A portion of this plasmid was derived from a plasmid made by: pRRL came from Naldini lab (JVI 98). R. Zufferey from Trono lab inserted the SIN in 98 and then WPRE in 99. Zennou et al finally inserted the cPPT.
• Articles Citing this Plasmid:
  • 159 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Please visit the Trono lab website http://tronolab.epfl.ch for cloning strategies, protocols, publications, and more.

Information for Cloning Grade DNA (Catalog # 12252-DNA.cg) ( Back to top)

Purpose

Cloning grade DNA is suitable for use in PCR, cloning reactions, or transformation into E. coli. The purity and amount is not suitable for direct transfections.

Delivery

• Amount: 2 µg
• Guaranteed Concentration: 100 ng/µl +/- 5 ng/µl
• Pricing: $105 USD
• Storage: DNA can be stored at 4℃ (short term) or -20℃ (long term).

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Quality Control

Addgene has verified this plasmid using Next Generation Sequencing. Results are available here