PL6-CS-WR
(Plasmid
#122512)
-
Purpose(Empty Backbone) construction for sgRNA transcription with Plasmodium falciparum U6 promotor
-
Depositing Lab
-
Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
---|---|---|---|---|---|
Plasmid | 122512 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
-
Vector backbonePL6
- Backbone size (bp) 6676
-
Vector typeCRISPR
- Promoter PfU6
-
Selectable markershDHFR
Growth in Bacteria
-
Bacterial Resistance(s)Ampicillin, 100 μg/mL
-
Growth Temperature37°C
-
Growth Strain(s)XL10 Gold
-
Copy numberHigh Copy
Cloning Information
- Cloning method Ligation Independent Cloning
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
-
For your Materials & Methods section:
PL6-CS-WR was a gift from Lubin Jiang (Addgene plasmid # 122512 ; http://n2t.net/addgene:122512 ; RRID:Addgene_122512) -
For your References section:
Epigenetic editing by CRISPR/dCas9 in Plasmodium falciparum. Xiao B, Yin S, Hu Y, Sun M, Wei J, Huang Z, Wen Y, Dai X, Chen H, Mu J, Cui L, Jiang L. Proc Natl Acad Sci U S A. 2019 Jan 2;116(1):255-260. doi: 10.1073/pnas.1813542116. Epub 2018 Dec 24. 10.1073/pnas.1813542116 PubMed 30584102