pUF1-dCas9-Sir2a
(Plasmid #122510)

• Purpose: Combining with the specific sgRNA sequence to repress the Plasmodium falciparum endogenous gene transcription, through the decreasing of H3K9 acetylation.
• Depositing Lab: Lubin Jiang
• Publication: Xiao et al Proc Natl Acad Sci U S A. 2019 Jan 2;116(1):255-260. doi: 10.1073/pnas.1813542116. Epub 2018 Dec 24.

Backbone

• Vector backbone: pUF1
• Backbone size w/o insert (bp): 11013
• Vector type: CRISPR
• Selectable markers: Blasticidin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): XL10 Gold
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: dCas9-Sir2a
• Alt name: dCas9-HDAC
• Species: Plasmodium falciparum
• Mutation: dCas9(D10A,H840A)
• Promoter: PfHsp86
• Tag / Fusion Protein:
  • 3*FLAG (N terminal on backbone)

Cloning Information

• Cloning method: Ligation Independent Cloning
• 5′ sequencing primer: Unknown

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Note: Plasmid contains a A250T mutation in Sir2a. This mutation is not known to affect plasmid function.