pCR2.1 Clover-LMNA Donor
(Plasmid #122508)

• Purpose: Homology repair template for in frame (first exon) clover knock-in of human LMNA gene
• Depositing Lab: Graham Dellaire
• Publication: Pinder et al Nucleic Acids Res. 2015 Oct 30;43(19):9379-92. doi: 10.1093/nar/gkv993. Epub 2015 Oct 1.

Backbone

• Vector backbone: pCR2.1 TOPO
• Backbone manufacturer: Invitrogen
• Backbone size w/o insert (bp): 3900
• Total vector size (bp): 5737
• Vector type: Homology Repair Template Plasmid (Donor Plasmid)

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Growth instructions: Kanamycin Resistance as well
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Homology Repair Template for Human LMNA (N-Terminal Clover Tag)
• Species: H. sapiens (human)
• Insert Size (bp): 1837
• Entrez Gene: LMNA (a.k.a. CDCD1, CDDC, CMD1A, CMT2B1, EMD2, FPL, FPLD, FPLD2, HGPS, IDC, LDP1, LFP, LGMD1B, LMN1, LMNC, LMNL1, MADA, PRO1)
• Tag / Fusion Protein:
  • Clover

Cloning Information

• Cloning method: TOPO Cloning
• 5′ sequencing primer: M13-Reverse
• 3′ sequencing primer: M13-Fwd

Resource Information

• Articles Citing this Plasmid:
  • 4 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry