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Addgene

pStrep-4xEGFP-N1
(Plasmid #122491)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 122491 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pEGFP-N1
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Strep-tagged 4xEGFP
  • Promoter CMV

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BspEI (destroyed during cloning)
  • 3′ cloning site NotI (not destroyed)
  • 5′ sequencing primer AGCTGGTTTAGTGAACCGTCA
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Please note: The MCS of Strep-tagged multimeric EGFP vectors differs from the MCS of the Strep-tagged monomeric EGFP vector.
pStrep-1xEGFP-N1:
G CTA GCG CTA CCG GAC TCA GAT CTC GAG CTC AAG CTT CGA ATT CTG CAG TCG ACG GTA CCG CGG GCC CGG GAT CCA GCC ACC ATG AGC GCT TGG AGC CAC CCG CAG TTC GAA AAA CGG GAT CCA CCG GTC GCC ACC ATG GTG
pStrep-2x/…/12xEGFP-N1:
G CTA GCG CTA CCG GAC TCA GAT CTC GAG CTC AAG CTT GAA ATC GAA TTC CCG CGG GCC CGG GAT CCA GCC ACC ATG AGC GCT TGG AGC CAC CCG CAG TTC GAA AAA CGG GAT CCA CCG GTC GCC ACC ATG GTG

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pStrep-4xEGFP-N1 was a gift from Georg Mayr (Addgene plasmid # 122491 ; http://n2t.net/addgene:122491 ; RRID:Addgene_122491)

  • For your References section:

    A toolkit for expression of Strep-tagged enhanced green fluorescent protein concatemers in mammalian cells. Zweifel A, Giehler S, Nalaskowski MM. Anal Biochem. 2019 Sep 12:113430. doi: 10.1016/j.ab.2019.113430. 10.1016/j.ab.2019.113430 PubMed 31521668
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