pLV-tTRKRAB
(Plasmid #12249)

• Depositing Lab: Didier Trono
• Publication: Wiznerowicz et al J Virol. 2003 Aug . 77(16):8957-61.

Backbone

• Vector backbone: pLV-tTRKRAB
• Backbone size w/o insert (bp): 10713
• Vector type: Mammalian Expression, Lentiviral, RNAi, Cre/Lox

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): Stbl3
• Growth instructions: Use Stbl3 or HB101 to reduce chance of recombination. Grow at 37C
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: TetR-KRAB fusion

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ sequencing primer: See map

Resource Information

• Addgene Notes:
  • Addgene diagnostic digest
• Articles Citing this Plasmid:
  • 6 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Older, double-copy vector system for conditional expression of siRNA. Has been replaced by the all-in-one vector system from Trono/Aebischer lab (e.g. see Addgene plasmid 11643).

For conditional expression of siRNA, the target cells need to be cotransduced with both pLVTH/pLVTHM and pLV-tTRKRAB/pLV-tTRKRAB-Red to achieve conditional expression of siRNA.

Please note that the full sequence for this plasmid is approximated and not fully verified. Please visit the Trono lab http://tronolab.epfl.ch for cloning strategies, protocols, publications, and more. See LentiWeb http://www.lentiweb.com for discussion on cloning strategies and protocols.