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PurposeA non-leaky heat-inducible gene expression system for Drosophila. (GenBank: MF058598.1)
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 122486 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepUASg
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Vector typeInsect Expression
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Selectable markersminiwhite
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Growth instructionsSee "Cloning and storage of PRExpress plasmid" document.
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Copy numberLow Copy
Gene/Insert 1
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Gene/Insert nameLacZ
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SpeciesE. coli
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Insert Size (bp)3144
Gene/Insert 2
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Gene/Insert namepoly-PRE
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SpeciesD. melanogaster (fly)
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Insert Size (bp)2447
Resource Information
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Supplemental Documents
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Articles Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
PRExpress was a gift from Renato Paro (Addgene plasmid # 122486 ; http://n2t.net/addgene:122486 ; RRID:Addgene_122486) -
For your References section:
Single vector non-leaky gene expression system for Drosophila melanogaster. Akmammedov A, Geigges M, Paro R. Sci Rep. 2017 Jul 31;7(1):6899. doi: 10.1038/s41598-017-07282-w. 10.1038/s41598-017-07282-w [pii] PubMed 28761084