HA-IFITM3-F8-TAG-C72A
(Plasmid #122477)

• Purpose: expresses human IFITM3-C72A with unnatural amino acid incorporated site specifically in response to the amber codon TAG in mammalian cells when co-transfected with tRNA synthetase/tRNA pair.
• Depositing Lab: Howard Hang
• Publication: Spence et al Nat Chem Biol. 2019 Jan 14. pii: 10.1038/s41589-018-0213-2. doi: 10.1038/s41589-018-0213-2.

Backbone

• Vector backbone: pCMV-HA
• Backbone manufacturer: clontech
• Backbone size w/o insert (bp): 3800
• Total vector size (bp): 4191
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: IFITM3
• Species: H. sapiens (human)
• Mutation: Changed Phenylalanine 8 to amber codon, cysteine 72 to alanine
• Entrez Gene: IFITM3 (a.k.a. 1-8U, DSPA2b, IP15)
• Tag / Fusion Protein:
  • HA (N terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: EcoRI (not destroyed)
• 3′ cloning site: SalI (not destroyed)
• 5′ sequencing primer: GATCCGGTACTAGAGGAACTGAAAAAC

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry