pcDNa3.1(+)_A009-H19
(Plasmid
#122473)
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Purposehuman H19 overexpression plasmid
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 122473 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepcDNA 3.1(+)
- Backbone size w/o insert (bp) 5428
- Total vector size (bp) 7762
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Vector typeMammalian Expression
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Selectable markersNeomycin (select with G418)
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameH19
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SpeciesH. sapiens (human)
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Insert Size (bp)2334
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GenBank IDNR_002196.2 NR_131223.1
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Entrez GeneH19 (a.k.a. ASM, ASM1, BWS, D11S813E, LINC00008, MIR675HG, NCRNA00008, WT2)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pcDNa3.1(+)_A009-H19 was a gift from Alexandra Kiemer (Addgene plasmid # 122473) -
For your References section:
The long non-coding RNA H19 suppresses carcinogenesis and chemoresistance in hepatocellular carcinoma. Schultheiss CS, Laggai S, Czepukojc B, Hussein UK, List M, Barghash A, Tierling S, Hosseini K, Golob-Schwarzl N, Pokorny J, Hachenthal N, Schulz M, Helms V, Walter J, Zimmer V, Lammert F, Bohle RM, Dandolo L, Haybaeck J, Kiemer AK, Kessler SM. Cell Stress. 2017 Aug 25;1(1):37-54. doi: 10.15698/cst2017.10.105. 10.15698/cst2017.10.105 PubMed 31225433