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Addgene

pcDNa3.1(+)_A009-H19
(Plasmid #122473)

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 122473 Standard format: Plasmid sent in bacteria as agar stab 1 $85

Backbone

  • Vector backbone
    pcDNA 3.1(+)
  • Backbone size w/o insert (bp) 5428
  • Total vector size (bp) 7762
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    H19
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    2334
  • GenBank ID
    NR_002196.2 NR_131223.1
  • Entrez Gene
    H19 (a.k.a. ASM, ASM1, BWS, D11S813E, LINC00008, MIR675HG, NCRNA00008, WT2)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pcDNa3.1(+)_A009-H19 was a gift from Alexandra Kiemer (Addgene plasmid # 122473)
  • For your References section:

    The long non-coding RNA H19 suppresses carcinogenesis and chemoresistance in hepatocellular carcinoma. Schultheiss CS, Laggai S, Czepukojc B, Hussein UK, List M, Barghash A, Tierling S, Hosseini K, Golob-Schwarzl N, Pokorny J, Hachenthal N, Schulz M, Helms V, Walter J, Zimmer V, Lammert F, Bohle RM, Dandolo L, Haybaeck J, Kiemer AK, Kessler SM. Cell Stress. 2017 Aug 25;1(1):37-54. doi: 10.15698/cst2017.10.105. 10.15698/cst2017.10.105 PubMed 31225433