pFRT/TO/GFP-SCAF4 (CRISPR_resistant)
(Plasmid
#122471)
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PurposeDox-inducible GFP (N-terminal tag) SCAF4 expression
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 122471 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepFRT/TO/GFP
- Backbone size w/o insert (bp) 6032
- Total vector size (bp) 9392
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Vector typeMammalian Expression
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Selectable markersHygromycin
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameSCAF4
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SpeciesH. sapiens (human)
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MutationSynonymous mutations conferring resistance to gRNAs described in Gregersen et al.
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GenBank IDNM_020706.2
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Entrez GeneSCAF4 (a.k.a. SFRS15, SRA4)
- Promoter CMV
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Tag
/ Fusion Protein
- GFP (N terminal on backbone)
Cloning Information
- Cloning method Gateway Cloning
- 5′ sequencing primer CMV forward
- 3′ sequencing primer BGH Reverse (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pFRT/TO/GFP-SCAF4 (CRISPR_resistant) was a gift from Jesper Svejstrup (Addgene plasmid # 122471 ; http://n2t.net/addgene:122471 ; RRID:Addgene_122471) -
For your References section:
SCAF4 and SCAF8, mRNA Anti-Terminator Proteins. Gregersen LH, Mitter R, Ugalde AP, Nojima T, Proudfoot NJ, Agami R, Stewart A, Svejstrup JQ. Cell. 2019 May 10. pii: S0092-8674(19)30457-X. doi: 10.1016/j.cell.2019.04.038. 10.1016/j.cell.2019.04.038 PubMed 31104839
