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pFRT/TO/FLAGHA-SCAF8
(Plasmid #122470)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 122470 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pFRT/TO/FLAGHA
  • Backbone size w/o insert (bp) 5294
  • Total vector size (bp) 9245
  • Vector type
    Mammalian Expression
  • Selectable markers
    Hygromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    SCAF8 isoform C CRISPR resistant
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    3951
  • Mutation
    Synonymous mutations conferring resistance to gRNAs described in Gregersen et al.
  • GenBank ID
    NM_001286189.1
  • Entrez Gene
    SCAF8 (a.k.a. RBM16)
  • Promoter CMV
  • Tag / Fusion Protein
    • FLAGHA (N terminal on backbone)

Cloning Information

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pFRT/TO/FLAGHA-SCAF8 was a gift from Jesper Svejstrup (Addgene plasmid # 122470 ; http://n2t.net/addgene:122470 ; RRID:Addgene_122470)

  • For your References section:

    SCAF4 and SCAF8, mRNA Anti-Terminator Proteins. Gregersen LH, Mitter R, Ugalde AP, Nojima T, Proudfoot NJ, Agami R, Stewart A, Svejstrup JQ. Cell. 2019 May 10. pii: S0092-8674(19)30457-X. doi: 10.1016/j.cell.2019.04.038. 10.1016/j.cell.2019.04.038 PubMed 31104839
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