pFRT/TO/SCAF4-FLAG
(Plasmid #122469)

• Purpose: Dox-inducible FLAG (C-terminal tag) SCAF4 expression
• Depositing Lab: Jesper Svejstrup
• Publication: Gregersen et al Cell. 2019 May 10. pii: S0092-8674(19)30457-X. doi: 10.1016/j.cell.2019.04.038.

Backbone

• Vector backbone: pFRT/TO
• Backbone size w/o insert (bp): 5081
• Total vector size (bp): 8600
• Vector type: Mammalian Expression
• Selectable markers: Hygromycin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: SCAF4
• Species: H. sapiens (human)
• Insert Size (bp): 3423
• GenBank ID: NM_020706.2
• Entrez Gene: SCAF4 (a.k.a. SFRS15, SRA4)
• Promoter: CMV
• Tag / Fusion Protein:
  • FLAG (C terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: KnpI (not destroyed)
• 3′ cloning site: NotI (not destroyed)
• 5′ sequencing primer: CMV forward
• 3′ sequencing primer: BGH Reverse

Resource Information

• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry