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Addgene

pDONR223 SCAF8_isoD_CRISPR_resistant
(Plasmid #122468)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 122468 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pDONR223
  • Backbone size w/o insert (bp) 2796
  • Total vector size (bp) 6612
  • Vector type
    Gateway cloning

Growth in Bacteria

  • Bacterial Resistance(s)
    Spectinomycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    SCAF8 isoform D CRISPR resistant
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    3816
  • Mutation
    Synonymous mutations conferring resistance to gRNAs described in Gregersen et al.
  • GenBank ID
    NM_014892.4
  • Entrez Gene
    SCAF8 (a.k.a. RBM16)

Cloning Information

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pDONR223 SCAF8_isoD_CRISPR_resistant was a gift from Jesper Svejstrup (Addgene plasmid # 122468 ; http://n2t.net/addgene:122468 ; RRID:Addgene_122468)

  • For your References section:

    SCAF4 and SCAF8, mRNA Anti-Terminator Proteins. Gregersen LH, Mitter R, Ugalde AP, Nojima T, Proudfoot NJ, Agami R, Stewart A, Svejstrup JQ. Cell. 2019 May 10. pii: S0092-8674(19)30457-X. doi: 10.1016/j.cell.2019.04.038. 10.1016/j.cell.2019.04.038 PubMed 31104839
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