pDONR223 SCAF8_isoD
(Plasmid
#122467)
-
PurposepDONR for Gateway cloning
-
Depositing Lab
-
Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
---|---|---|---|---|---|
Plasmid | 122467 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
-
Vector backbonepDONR223
- Backbone size w/o insert (bp) 2796
- Total vector size (bp) 6612
-
Vector typeGateway cloning
Growth in Bacteria
-
Bacterial Resistance(s)Spectinomycin, 50 μg/mL
-
Growth Temperature37°C
-
Growth Strain(s)DH5alpha
-
Copy numberHigh Copy
Gene/Insert
-
Gene/Insert nameSCAF8 isoform D
-
SpeciesH. sapiens (human)
-
Insert Size (bp)3816
-
GenBank IDNM_014892
-
Entrez GeneSCAF8 (a.k.a. RBM16)
Cloning Information
- Cloning method Gateway Cloning
- 5′ sequencing primer M13 forward
- 3′ sequencing primer M13 reverse (Common Sequencing Primers)
Resource Information
-
Supplemental Documents
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
-
For your Materials & Methods section:
pDONR223 SCAF8_isoD was a gift from Jesper Svejstrup (Addgene plasmid # 122467 ; http://n2t.net/addgene:122467 ; RRID:Addgene_122467) -
For your References section:
SCAF4 and SCAF8, mRNA Anti-Terminator Proteins. Gregersen LH, Mitter R, Ugalde AP, Nojima T, Proudfoot NJ, Agami R, Stewart A, Svejstrup JQ. Cell. 2019 May 10. pii: S0092-8674(19)30457-X. doi: 10.1016/j.cell.2019.04.038. 10.1016/j.cell.2019.04.038 PubMed 31104839