pLOX-Ttag-iresTK
(Plasmid #12246)

• Depositing Lab: Didier Trono
• Publication: Salmon et al Mol Ther. 2000 Oct . 2(4):404-14.

Backbone

• Vector backbone: pHR'
• Backbone size w/o insert (bp): 12290
• Vector type: Mammalian Expression, Lentiviral, Cre/Lox

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): Stbl3
• Growth instructions: Use Stbl3 or HB101 to reduce chance of recombination. Grow at 37C
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: SV40 Large T, HSV1-TK
• Alt name: SV40 Large T
• Alt name: HSV-1 thymidine kinase
• Species: Simian virus
• Mutation: LoxP in 3' LTR.
• Entrez Gene: SV40gp6 (a.k.a. SV40gp6)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ sequencing primer: CMV Fwd for LargeT
• 3′ sequencing primer: SP6 primer rev for LoxP

Resource Information

• Articles Citing this Plasmid:
  • 24 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

CMV promoter is upstream of the bicistronic coding cassette with SV40-Large T and HSV1-TK. During reverse transcription U3 region of the 3'LTR, with LoxP, is duplicated; LoxP sites end up flanking the genome of the integrated provirus. Upon expression of Cre, provirus is excised.

Please note that the full sequence for this plasmid is approximated and not fully verified--there will be discrepancies between Addgene's quality control sequence and the theoretical sequence from the depositor. Please visit the Trono lab http://tronolab.epfl.ch for cloning strategies, protocols, publications, and more. See LentiWeb http://www.lentiweb.com for discussion on cloning strategies and protocols.