huβB1cry:mAppleCAAX
(Plasmid #122451)

• Purpose: Human BB1 crystallin promoter driving mApple-CAAX in Tol2 destination vector
• Depositing Lab: Thomas Schilling
• Publication: Vorontsova et al J Vis Exp. 2019 May 6;(147). doi: 10.3791/59528.

Backbone

• Vector backbone: pDestTol2pA2
• Backbone manufacturer: Tol2 Kit
• Backbone size w/o insert (bp): 4445
• Total vector size (bp): 5540
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Human BB1 crystallin:mApple_CAAX
• Species: H. sapiens (human)
• Insert Size (bp): 1095
• GenBank ID: NG_009826
• Promoter: 300bp Human Beta B1 crystallin promoter

Cloning Information

• Cloning method: Gateway Cloning
• 5′ sequencing primer: M13R

Resource Information

• Supplemental Documents:
  • pIV3-pDestTol2pA2-HuBb1cry-mAppleCAAX.gb
• A portion of this plasmid was derived from a plasmid made by: We obtained the 300bp human BB1 crystallin promoter fragment from Bei-En Chang's laboratory at the National Taiwan University. Hou, H.-H., Kuo, M. Y.-P., Luo, Y.-W. & Chang, B.-E. Recapitulation of human βB1-crystallin promoter activity in transgenic zebrafish. Developmental Dynamics. 235 (2), 435-443, (2006). We obtained pDestTol2pA2 (#394), and p3E-polyA (#302) from the Tol2kit v1.2, and pMEmAppleCAAX (#768) from Tol2kit v2.0 (Kristen Kwan lab University of Utah). Kwan, K. M. et al. The Tol2kit: A multisite gateway-based construction kit for Tol2 transposon transgenesis constructs. Developmental Dynamics. 236 (11), 3088-3099, (2007).

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • National Institute of Genetics Limited Use Label License for Tol2 System
• Industry Terms:
  • Not Available to Industry

Depositor Comments

You can access the article associated with this plasmid on JOVE at the following url:https://www.jove.com/video/59528/assessment-zebrafish-lens-nucleus-localization-sutural