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rat brain inositol 1,4,5-trisphosphate 3-kinase A (Clone C5, partial sequence from V66)
(Plasmid #122450)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 122450 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pBluescript
  • Backbone manufacturer
    Stratagene
  • Total vector size (bp) 2900
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    inositol 1,4,5-trisphosphate 3-kinase A
  • Alt name
    ITPKA
  • Species
    R. norvegicus (rat)
  • Insert Size (bp)
    1600
  • Mutation
    coding sequence from V66 till stop codon
  • GenBank ID
    X56917.1
  • Entrez Gene
    Itpka
  • Tag / Fusion Protein
    • beta-galactosidase (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRI (not destroyed)
  • 3′ cloning site EcoRI (not destroyed)
  • 5′ sequencing primer M13 forward primer
  • 3′ sequencing primer M13 reverse primer
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Note: Addgene NGS unable to fully confirm the length of the poly-A tail on the inositol 1,4,5-trisphosphate 3-kinase A insert sequence. This is not known to affect plasmid function.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    rat brain inositol 1,4,5-trisphosphate 3-kinase A (Clone C5, partial sequence from V66) was a gift from Christophe Erneux (Addgene plasmid # 122450 ; http://n2t.net/addgene:122450 ; RRID:Addgene_122450)

  • For your References section:

    Cloning and expression in Escherichia coli of a rat brain cDNA encoding a Ca2+/calmodulin-sensitive inositol 1,4,5-trisphosphate 3-kinase. Takazawa K, Vandekerckhove J, Dumont JE, Erneux C. Biochem J. 1990 Nov 15;272(1):107-12. PubMed 2176078
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