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pZ149-pcDNA3-BhCas12b_v4
(Plasmid #122446)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 122446 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pcDNA3.1
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    CMV-NLS-BhCas12b_v4-NLS-3xHA

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pZ149-pcDNA3-BhCas12b_v4 was a gift from Feng Zhang (Addgene plasmid # 122446 ; http://n2t.net/addgene:122446 ; RRID:Addgene_122446)

  • For your References section:

    Engineering of CRISPR-Cas12b for human genome editing. Strecker J, Jones S, Koopal B, Schmid-Burgk J, Zetsche B, Gao L, Makarova KS, Koonin EV, Zhang F. Nat Commun. 2019 Jan 22;10(1):212. doi: 10.1038/s41467-018-08224-4. 10.1038/s41467-018-08224-4 PubMed 30670702
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