pZ143-pcDNA3-BvCas12b
(Plasmid
#122445)
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PurposeExpresses BvCas12b in mammalian cells
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 122445 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepcDNA3.1
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameCMV-NLS-BvCas12b-NLS-3xHA
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Tag
/ Fusion Protein
- 3XHA (C terminal on insert)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pZ143-pcDNA3-BvCas12b was a gift from Feng Zhang (Addgene plasmid # 122445 ; http://n2t.net/addgene:122445 ; RRID:Addgene_122445) -
For your References section:
Engineering of CRISPR-Cas12b for human genome editing. Strecker J, Jones S, Koopal B, Schmid-Burgk J, Zetsche B, Gao L, Makarova KS, Koonin EV, Zhang F. Nat Commun. 2019 Jan 22;10(1):212. doi: 10.1038/s41467-018-08224-4. 10.1038/s41467-018-08224-4 PubMed 30670702
