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Addgene

pCrlM
(Plasmid #122444)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 122444 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pCrl
  • Backbone manufacturer
    Curran lab (Addgene plasmid 122443)
  • Total vector size (bp) 16000
  • Modifications to backbone
    Myc epitope tag 9E10 (QKLISEEDLN) was inserted into the unique BspEI site present in pCrl
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    reelin
  • Species
    M. musculus (mouse)
  • Insert Size (bp)
    10383
  • GenBank ID
    U24703.1
  • Entrez Gene
    Reln (a.k.a. reeler, rl)
  • Promoter CMV
  • Tag / Fusion Protein
    • Myc

Cloning Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCrlM was a gift from Tom Curran (Addgene plasmid # 122444 ; http://n2t.net/addgene:122444 ; RRID:Addgene_122444)

  • For your References section:

    Reelin is a secreted glycoprotein recognized by the CR-50 monoclonal antibody. D'Arcangelo G, Nakajima K, Miyata T, Ogawa M, Mikoshiba K, Curran T. J Neurosci. 1997 Jan 1;17(1):23-31. PubMed 8987733
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