Addgene: pLOX-CWBmi1 Skip to main content
Addgene

pLOX-CWBmi1
(Plasmid #12240)

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 12240 Standard format: Plasmid sent in bacteria as agar stab 1 $85

Backbone

  • Vector backbone
    pHR
  • Backbone size w/o insert (bp) 9967
  • Vector type
    Mammalian Expression, Lentiviral, Cre/Lox

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    Stbl3
  • Growth instructions
    Use Stbl3 or HB101 to reduce chance of recombination. Grow at 37C
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Bmi1
  • Species
    M. musculus (mouse)
  • Mutation
    CMV promoter upstream of mBmi-1. LoxP in 3' LTR.
  • Entrez Gene
    Bmi1 (a.k.a. Bmi-1, Pcgf4)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ sequencing primer CMV fwd
  • 3′ sequencing primer SP6 for LoxP site
  • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Please note that the full sequence for this plasmid is approximated and not fully verified. Please visit the Trono lab http://tronolab.epfl.ch for cloning strategies, protocols, publications, and more. See LentiWeb http://www.lentiweb.com for discussion on cloning strategies and protocols.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pLOX-CWBmi1 was a gift from Didier Trono (Addgene plasmid # 12240 ; http://n2t.net/addgene:12240 ; RRID:Addgene_12240)
  • For your References section:

    Reversible immortalization of human primary cells by lentivector-mediated transfer of specific genes. Salmon P, Oberholzer J, Occhiodoro T, Morel P, Lou J, Trono D. Mol Ther. 2000 Oct . 2(4):404-14. 10.1006/mthe.2000.0141 PubMed 11020357