pLOX-CWBmi1
(Plasmid #12240)

• Depositing Lab: Didier Trono
• Publication: Salmon et al Mol Ther. 2000 Oct . 2(4):404-14.

Backbone

• Vector backbone: pHR
• Backbone size w/o insert (bp): 9967
• Vector type: Mammalian Expression, Lentiviral, Cre/Lox

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): Stbl3
• Growth instructions: Use Stbl3 or HB101 to reduce chance of recombination. Grow at 37C
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Bmi1
• Species: M. musculus (mouse)
• Mutation: CMV promoter upstream of mBmi-1. LoxP in 3' LTR.
• Entrez Gene: Bmi1 (a.k.a. Bmi-1, Pcgf4)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ sequencing primer: CMV fwd
• 3′ sequencing primer: SP6 for LoxP site

Resource Information

• Articles Citing this Plasmid:
  • 11 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Please note that the full sequence for this plasmid is approximated and not fully verified. Please visit the Trono lab http://tronolab.epfl.ch for cloning strategies, protocols, publications, and more. See LentiWeb http://www.lentiweb.com for discussion on cloning strategies and protocols.