p316CUPSup35GFPSG
(Plasmid
#1224)
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Depositing Lab
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 1224 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonep316Cup1
- Backbone size w/o insert (bp) 5299
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Vector typeYeast Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameSUP35
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SpeciesS. cerevisiae (budding yeast)
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Insert Size (bp)2789
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GenBank IDAY553990
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Entrez GeneSUP35 (a.k.a. YDR172W, GST1, PNM2, SAL3, SUF12, SUP2, SUP36)
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Tag
/ Fusion Protein
- GFPSG S65T V164A (C terminal on insert)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site BamH1 (not destroyed)
- 3′ cloning site Sac2 (not destroyed)
- 5′ sequencing primer n/a (Common Sequencing Primers)
Resource Information
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Article Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
p316CUPSup35GFPSG was a gift from Susan Lindquist (Addgene plasmid # 1224 ; http://n2t.net/addgene:1224 ; RRID:Addgene_1224) -
For your References section:
Changes in the middle region of Sup35 profoundly alter the nature of epigenetic inheritance for the yeast prion [PSI+]. Liu JJ, Sondheimer N, Lindquist SL. Proc Natl Acad Sci U S A 2002 Dec 10;99 Suppl 4:16446-53. Epub 2002 Dec 02. 10.1073/pnas.252652099 PubMed 12461168