pLOX-CW-CRE
(Plasmid #12238)

• Depositing Lab: Didier Trono
• Publication: Cudre-Mauroux et al Hum Gene Ther. 2003 Nov 1. 14(16):1525-33.

Backbone

• Vector backbone: pHR
• Backbone size w/o insert (bp): 9886
• Vector type: Mammalian Expression, Lentiviral, Cre/Lox

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): Stbl3
• Growth instructions: Use Stbl3 or HB101 to reduce chance of recombination. Grow at 37C
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: CRE
• Mutation: CMV promoter is upstream of nls-CRE. LoxP in 3' LTR.

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ sequencing primer: CMV forward
• 3′ sequencing primer: SP6 for lox site

Resource Information

• Addgene Notes:
  • Sequence with SP6 primer
  • Restriction digest of pLOX-CW-CRE
  • Addgene diagnostic digest
• Articles Citing this Plasmid:
  • 2 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Please note that the full sequence for this plasmid is approximated and not fully verified. ALTHOUGH THE SEQUENCE OF THIS PLASMID SUGGESTS IT'S FULL SIZE IS 9.9KB, OUR TEST DIGEST SHOWS IT IS ACTUALLY 11.5KB. NONETHELESS, OUR CUSTOMERS HAVE BEEN ABLE TO MAKE FUNCTIONAL VIRAL PARTICLES USING THIS PLASMID.

Please visit the Trono lab http://tronolab.epfl.ch for cloning strategies, protocols, publications, and more. See LentiWeb http://www.lentiweb.com for discussion on cloning strategies and protocols.