pRS143
(Plasmid
#122377)
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Purpose(Empty Backbone) CRISPRi plasmid for use in Candida albicans. Contains dCas9 and NEUT5L integration site and the sgRNA cloning site (SNR52 promoter, PacI sites, sgRNA tail)
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 122377 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepJK-caCas9-NatMX-Neut5L
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Vector typeYeast Expression, CRISPR
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Selectable markersNAT
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pRS143 was a gift from Rebecca Shapiro (Addgene plasmid # 122377 ; http://n2t.net/addgene:122377 ; RRID:Addgene_122377) -
For your References section:
A CRISPR Interference Platform for Efficient Genetic Repression in Candida albicans. Wensing L, Sharma J, Uthayakumar D, Proteau Y, Chavez A, Shapiro RS. mSphere. 2019 Feb 13;4(1). pii: 4/1/e00002-19. doi: 10.1128/mSphere.00002-19. 10.1128/mSphere.00002-19 PubMed 30760609
Map uploaded by the depositor.
