Skip to main content
Addgene

pLR5-CBh-dCas9-hEzh2-IRES-Hyg
(Plasmid #122375)

Print

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 122375 Standard format: Plasmid sent in bacteria as agar stab 1 $85
Add to Cart

Backbone

  • Vector backbone
    pPB-LR5
  • Backbone manufacturer
    Allan Bradley
  • Backbone size w/o insert (bp) 3554
  • Total vector size (bp) 13240
  • Vector type
    Mammalian Expression ; piggyBac

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    CBh-dCas9-hEzh2-IRES-Hyg-pA
  • Species
    Synthetic
  • Insert Size (bp)
    9686

Cloning Information

Resource Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pLR5-CBh-dCas9-hEzh2-IRES-Hyg was a gift from Hiroshi Ochiai (Addgene plasmid # 122375 ; http://n2t.net/addgene:122375 ; RRID:Addgene_122375)

  • For your References section:

    Genome-wide kinetic properties of transcriptional bursting in mouse embryonic stem cells. Ochiai H, Hayashi T, Umeda M, Yoshimura M, Harada A, Shimizu Y, Nakano K, Saitoh N, Liu Z, Yamamoto T, Okamura T, Ohkawa Y, Kimura H, Nikaido I. Sci Adv. 2020 Jun 17;6(25):eaaz6699. doi: 10.1126/sciadv.aaz6699. eCollection 2020 Jun. 10.1126/sciadv.aaz6699 PubMed 32596448
Related items:
Commonly requested with: