pTV-Trim28-tdiRFP670-PEST
(Plasmid
#122367)
-
PurposeTargeting vector for mouse Trim28 to insert 2A-tdiRFP670-PEST into immediately upstream of stop codon.
-
Depositing Lab
-
Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
---|---|---|---|---|---|
Plasmid | 122367 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
-
Vector backbonepBSK
- Backbone size w/o insert (bp) 2850
- Total vector size (bp) 7136
-
Vector typeMouse Targeting
Growth in Bacteria
-
Bacterial Resistance(s)Ampicillin, 100 μg/mL
-
Growth Temperature37°C
-
Growth Strain(s)DH5alpha
-
Copy numberHigh Copy
Gene/Insert
-
Gene/Insert name2A-tdiRFP-PEST with homology arms for Trim28
-
SpeciesSynthetic
-
Insert Size (bp)4286
Cloning Information
- Cloning method Gibson Cloning
- 5′ sequencing primer M13-fwr
- 3′ sequencing primer M13-rev (Common Sequencing Primers)
Resource Information
-
Supplemental Documents
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
-
For your Materials & Methods section:
pTV-Trim28-tdiRFP670-PEST was a gift from Hiroshi Ochiai (Addgene plasmid # 122367 ; http://n2t.net/addgene:122367 ; RRID:Addgene_122367) -
For your References section:
Genome-wide kinetic properties of transcriptional bursting in mouse embryonic stem cells. Ochiai H, Hayashi T, Umeda M, Yoshimura M, Harada A, Shimizu Y, Nakano K, Saitoh N, Liu Z, Yamamoto T, Okamura T, Ohkawa Y, Kimura H, Nikaido I. Sci Adv. 2020 Jun 17;6(25):eaaz6699. doi: 10.1126/sciadv.aaz6699. eCollection 2020 Jun. 10.1126/sciadv.aaz6699 PubMed 32596448